8PJ7
MLLT3 in complex with compound PFI-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-09-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 49.632, 49.632, 122.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.000 - 1.260 |
| R-factor | 0.19876 |
| Rwork | 0.198 |
| R-free | 0.22171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.865 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.305 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.074 | 1.597 |
| Rmeas | 0.076 | |
| Rpim | 0.014 | 0.482 |
| Number of reflections | 42219 | 4068 |
| <I/σ(I)> | 20.71 | 1.27 |
| Completeness [%] | 99.8 | |
| Redundancy | 27.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Bis-Tris pH=7.0 150 mM AmSO4 Gradient 20-30% PEG3350 |
