8PDE
Crystal Structure of the MADS-box/MEF2 Domain of MEF2D bound to dsDNA and HDAC4 deacetylase binding motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-11 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.024, 76.330, 56.240 |
Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
Resolution | 45.650 - 2.400 |
Rwork | 0.217 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.650 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.132 | 0.865 |
Number of reflections | 17911 | 1938 |
<I/σ(I)> | 7 | 1.7 |
Completeness [%] | 94.7 | |
Redundancy | 4.1 | |
CC(1/2) | 0.992 | 0.533 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium formate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350 |