8PC2

SelDeg51 in complex with FKBP51FK1 domain and pVHL:EloB:EloC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.918400
Spacegroup name	I 1 2 1
Unit cell lengths	138.646, 68.425, 159.256
Unit cell angles	90.00, 114.45, 90.00

Refinement procedure

Resolution	48.739 - 2.800
Rwork	0.210
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.581
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.740	48.740	2.940
High resolution limit [Å]	2.800	9.290	2.800
Rmerge	0.088	0.028	0.523
Rmeas	0.113	0.036	0.668
Rpim	0.070	0.022	0.409
Number of reflections	33478	946	4459
<I/σ(I)>	12
Completeness [%]	99.1
Redundancy	4.6	4	4.7
CC(1/2)	0.997	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	15% PEG3350, 0.2 M tri-sodium citrate, 0.1 M HEPES-NaOH pH 7.5 and 10% glycol