8OZP
In situ subtomogram average of Prototype Foamy Virus Env pentamer of trimers
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 11.9 |
| Software | Dynamo,RELION,FEI tomography,Gctf,ISOLDE,REFMAC |
| Symmetry | C5 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
