8OZL
In situ cryoEM structure of the Prototype Foamy Virus capsid, pentamer localised reconstruction
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum LS |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.4 |
Number of Particle | 52141 |
Software | crYOLO,RELION,EPU,Gctf,REFMAC |
Symmetry | C5 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |