8OXU
Crystal Structure of the Hsp90-LA1011 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96864 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.714, 97.714, 275.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.710 - 2.940 |
| R-factor | 0.2498 |
| Rwork | 0.247 |
| R-free | 0.30620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.051 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.100 | 3.190 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Number of reflections | 23054 | 1153 |
| <I/σ(I)> | 9 | 1.4 |
| Completeness [%] | 91.9 | 58.1 |
| Redundancy | 9.2 | 4 |
| CC(1/2) | 0.995 | 0.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | MES pH 7.5, Magnesium chloride hexahydrate, PEG Smear Medium, 2propanol |
