Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.080, 66.140, 107.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.080 - 1.800 |
| R-factor | 0.1625 |
| Rwork | 0.161 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.141 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASES |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.080 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 40369 | 3515 |
| <I/σ(I)> | 31.18 | |
| Completeness [%] | 98.4 | |
| Redundancy | 12.8 | |
| CC(1/2) | 1.000 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 8.25-8.75 0.1 M Magnesium acetate 15-18% PEG 3350 |
