8OVH
Crystal structure of O-acetyl-L-homoserine sulfhydrolase from Saccharomyces cerevisiae in complex with Pyridoxal-5'-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.579, 118.218, 131.381 |
| Unit cell angles | 90.00, 92.91, 90.00 |
Refinement procedure
| Resolution | 49.590 - 2.171 |
| Rwork | 0.172 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.592 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.590 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.180 | 1.600 |
| Number of reflections | 629183 | 3958 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 97.5 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 22% (w/v) PEG 6000, 200 mM LiCl, and 100 mM sodium acetate, pH 5.0 |
