8ONV
KRAS-G13D in complex with BI-2493
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-24 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.799, 40.845, 55.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.880 - 1.010 |
| R-factor | 0.1347 |
| Rwork | 0.134 |
| R-free | 0.15650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 1.097 |
| High resolution limit [Å] | 1.010 | 1.011 |
| Number of reflections | 83260 | 4164 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 93.1 | |
| Redundancy | 11.8 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.2mM MgCl2, 15-27% PEG 2000, 100mM sodium acetate pH=4.4 |
