8OM6
Crystal structure of FGF2-STAB, a stable variant of human fibroblast growth factor 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-06 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.510, 38.670, 34.260 |
| Unit cell angles | 90.00, 104.64, 90.00 |
Refinement procedure
| Resolution | 33.148 - 1.310 |
| R-factor | 0.1982 |
| Rwork | 0.197 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.799 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.148 | 1.357 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmeas | 0.043 | 1.216 |
| Number of reflections | 35777 | 3496 |
| <I/σ(I)> | 15.28 | 0.75 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292.15 | PEG 3350, Bis-Tris |
