8OEE

Crystal structure of human AQP2 T126M mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 42
Unit cell lengths	118.940, 118.940, 89.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.750 - 3.150
R-factor	0.252
Rwork	0.250
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.980	3.360
High resolution limit [Å]	3.150	3.150
Number of reflections	42680	3952
<I/σ(I)>	13.1
Completeness [%]	99.8
Redundancy	11.3
CC(1/2)	0.998	0.208

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20- 30% PEG400, Tris-HCl pH 8.5, 0.1M MgCl2, 0.1M NaCl, 0.025 M CdCl2 added to the drop only