8ODO

Structure of human guanylylated RTCB in complex with Archease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9737
Spacegroup name	P 1 21 1
Unit cell lengths	99.090, 125.547, 125.733
Unit cell angles	90.00, 108.51, 90.00

Refinement procedure

Resolution	75.230 - 2.200
R-factor	0.1945
Rwork	0.193
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292120363
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	XDS (20210205)
Data scaling software	Aimless (1.12.10)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.230	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.110	1.160
Rpim	0.045	0.495
Number of reflections	145859	6868
<I/σ(I)>	9.2	1.1
Completeness [%]	99.3	95.2
Redundancy	7	6.3
CC(1/2)	0.998	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	protein: RTCB 160 uM, Archease 200 uM, 2 mM manganese chloride, 2 mM GTP reservoir: 10% w/v PEG 8000, 0.1 M HEPES pH 7.5, 8% v/v ethylene glycol drop: 200 nl protein + 200 nl reservoir