8KH8

Crystal structure of FGFR4(V550L) kinase domain with 8z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	277
Detector technology	CCD
Collection date	2023-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	42.141, 61.457, 60.417
Unit cell angles	90.00, 97.82, 90.00

Refinement procedure

Resolution	36.660 - 1.490
R-factor	0.1693
Rwork	0.168
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wcw
RMSD bond length	0.008
RMSD bond angle	1.035
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.460	1.530
High resolution limit [Å]	1.490	1.490
Number of reflections	49894	3623
<I/σ(I)>	7.8
Completeness [%]	99.9
Redundancy	6.3
CC(1/2)	0.993	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19% PEG3350