8JYR
Crystal structure of anti-HER2 antibody H2Mab-119 in complex with HER2 domain I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.979, 112.291, 60.537 |
| Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
| Resolution | 40.020 - 1.690 |
| R-factor | 0.1752 |
| Rwork | 0.173 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 1.800 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 79311 | 12651 |
| <I/σ(I)> | 9.46 | 1.35 |
| Completeness [%] | 99.1 | 97.9 |
| Redundancy | 3.52 | 3.6 |
| CC(1/2) | 0.997 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium citrate pH5.5, 20% w/v PEG3000 |
