8JYC
Crystal Structure of Intracellular B30.2 Domain of BTN3A1 and BTN2A1 in Complex with DMAPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 |
| Unit cell lengths | 90.153, 90.153, 167.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.600 - 2.290 |
| R-factor | 0.1731 |
| Rwork | 0.171 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zxk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.713 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.370 |
| High resolution limit [Å] | 2.290 | 4.930 | 2.290 |
| Rmerge | 0.119 | 0.048 | 0.729 |
| Rmeas | 0.123 | 0.050 | 0.758 |
| Rpim | 0.033 | 0.014 | 0.207 |
| Total number of observations | 825425 | ||
| Number of reflections | 60267 | 6114 | 6050 |
| <I/σ(I)> | 4 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 13.7 | 13.4 | 13.4 |
| CC(1/2) | 0.999 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 42% PEG200, 0.1 M HEPES pH7.5 |
