8JNA

CRAF ras-binding domain chimera, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	39.696, 58.744, 69.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.530 - 1.700
R-factor	0.2527
Rwork	0.252
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.491
Data reduction software	XDS (Feb 3, 2021)
Data scaling software	XDS (Feb 3, 2021)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.530	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.041	0.706
Rmeas	0.045	0.804
Number of reflections	9147	866
<I/σ(I)>	21.1	1.59
Completeness [%]	98.8	95.65
Redundancy	6.7	4.4
CC(1/2)	1.000	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1.0M tri-sodium citrate, 0.1M imidazole, pH 8.0