8JI8
Crystal Structure of Prophenoloxidase PPO6 chimeric mutant (F215EASNRAIVD224 to G215DGPDSVVR223) from Aedes aegypti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.570, 153.660, 223.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.690 - 2.650 |
| R-factor | 0.18018 |
| Rwork | 0.176 |
| R-free | 0.26133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MR-Rosetta |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.160 | 0.790 |
| Rmeas | 0.173 | 0.907 |
| Rpim | 0.066 | 0.436 |
| Number of reflections | 90756 | 3878 |
| <I/σ(I)> | 12.2 | 2.1 |
| Completeness [%] | 98.6 | 86.3 |
| Redundancy | 12.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.1 M imidazole pH7.0, 2% v/v Tacsimate pH7.0, 5% v/v 2-ProOH, 6%-10% v/v PEG 3350, |
