8JC5
Crystal structure of PLEKHM1 RUN domain in complex with GTP-bound Arl8b(Q75L)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97873 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.976, 73.817, 91.959 |
Unit cell angles | 90.00, 114.18, 90.00 |
Refinement procedure
Resolution | 46.750 - 2.010 |
R-factor | 0.1686 |
Rwork | 0.167 |
R-free | 0.20090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.205 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.890 | 2.040 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.047 | 0.573 |
Number of reflections | 63813 | 63813 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 96.9 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Lithium chloride, 0.1 M TRIS pH=7.6, 18% (w/v) PEG 8000 |