8J6K
Crystal structure of pro-interleukin-18 and caspase-4 complex
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-12 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 86.254, 86.254, 194.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 59.200 - 3.120 |
R-factor | 0.2411 |
Rwork | 0.238 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.700 | 3.290 |
High resolution limit [Å] | 3.120 | 3.120 |
Rmerge | 0.113 | 1.312 |
Rmeas | 0.119 | |
Rpim | 0.035 | 0.410 |
Number of reflections | 15561 | 2223 |
<I/σ(I)> | 16 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.1 | 11.2 |
CC(1/2) | 0.999 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Calcium acetate, 0.1M Tris-HCl pH 9.0, 13% PEG 8000, 0.01 M L-Glutathione reduced, 0.01 M L-Glutathione oxidized |