8IZC
Human CK1 Delta Kinase structure bound to Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.999, 48.950, 82.052 |
| Unit cell angles | 95.75, 95.25, 106.12 |
Refinement procedure
| Resolution | 14.620 - 1.450 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in-house structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 14.620 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.036 | 0.036 |
| Number of reflections | 113119 | 112996 |
| <I/σ(I)> | 23 | |
| Completeness [%] | 96.1 | 96.1 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M SODIUM SULPHATE, 0.100 M SODIUM CITRATE PH 5.5 , 18% PEG3350 |
