8IVU
Crystal Structure of Human NAMPT in complex with A4276
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.560, 116.690, 195.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.708 - 2.090 |
R-factor | 0.205423269196 |
Rwork | 0.204 |
R-free | 0.23133 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.580 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1-2575-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.710 | 2.150 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmeas | 0.203 | |
Number of reflections | 132238 | 12302 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.9 | |
Redundancy | 13.8 | |
CC(1/2) | 0.594 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 283.15 | 0.1M sodium phosphate pH 7.0, 0.2M sodium chloride, 25% (w/v) polyethylene glycol (PEG) 3350 |