8ISN

HLA-A24 in complex with modified 9mer WT1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	F 2 2 2
Unit cell lengths	161.400, 166.130, 180.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.240 - 2.480
R-factor	0.2241
Rwork	0.222
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.511
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.630
High resolution limit [Å]	2.480	2.480
Number of reflections	42937	6885
<I/σ(I)>	11.8	0.97
Completeness [%]	100.0
Redundancy	18
CC(1/2)	0.997	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium tartrate dibasic, 20 % (w/v) PEG 3350