8ILH
The crystal structure of dG(Se-Sp)-DNA:Pol X product binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.471, 87.280, 87.056 |
Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.18306 |
Rwork | 0.181 |
R-free | 0.22730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.403 |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.078 | 0.047 | 0.237 |
Total number of observations | 255533 | ||
Number of reflections | 51431 | 5393 | 4593 |
<I/σ(I)> | 8.2 | ||
Completeness [%] | 96.9 | 99.3 | 87.2 |
Redundancy | 5 | 6.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | ammonium acetate (0.2 M), HEPES pH 7.2 (0.1 M), 25% PEG3350 |