8IFO

Crystal structure of estrogen related receptor-gamma DNA binding domain complexed with Pla2g12b promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	77.403, 74.434, 111.020
Unit cell angles	90.00, 104.98, 90.00

Refinement procedure

Resolution	44.290 - 2.200
R-factor	0.22041
Rwork	0.219
R-free	0.26001
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.651
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.620	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.084	0.509
Rmeas	0.091	0.557
Rpim	0.036	0.223
Total number of observations	199080	16364
Number of reflections	30806	2668
<I/σ(I)>	9.8	2.7
Completeness [%]	99.2
Redundancy	6.5	6.1
CC(1/2)	0.994	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Sodium malonate pH 6.0, 12% w/v Polyethylene glycol 3,350