8ID2
Crystal structure of the ubiquitin-like domain in the SF3A1 subunit of human U2 snRNP complexed with the stem-loop 4 of U1 snRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-04 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.07000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.455, 60.214, 103.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.390 - 1.800 |
R-factor | 0.1965 |
Rwork | 0.194 |
R-free | 0.23740 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.254 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHARP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.390 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.049 | 4.198 |
Rmeas | 0.051 | 4.362 |
Rpim | 0.014 | 1.604 |
Number of reflections | 32309 | 1809 |
<I/σ(I)> | 20.5 | |
Completeness [%] | 99.7 | 95.8 |
Redundancy | 13.4 | 13.4 |
CC(1/2) | 0.999 | 0.458 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM MIB buffer (pH 5.0), 25% PEG 1500 |