8I0B
The crystal structure of human glutamate receptor 2 in complex with LT-102
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 121.400, 47.104, 98.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.490 - 1.730 |
| R-factor | 0.2225 |
| Rwork | 0.221 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 6.648 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.410 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.097 | |
| Number of reflections | 59162 | 8326 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 98.8 | |
| Redundancy | 12 | |
| CC(1/2) | 0.999 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG 8000, 0.1M NaAC pH 5.0, 0.2M (NH4)2SO4 |
