8HUE
Crystal structure of FGF2-M2 mutant - D28E/C78I/C96I/S137P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.381, 54.446, 71.968 |
| Unit cell angles | 90.00, 101.46, 90.00 |
Refinement procedure
| Resolution | 45.480 - 1.480 |
| Rwork | 0.179 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.071 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.480 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.105 | |
| Number of reflections | 64285 | 3196 |
| <I/σ(I)> | 26.1 | 3.9 |
| Completeness [%] | 98.3 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.986 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 289.15 | 30% (w/v) polyethylene glycol 2000, 100mM potassium thiocyanate |
