8HTG
Crystal structure of Golf in complex with GTP-gamma S and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.730, 52.360, 125.510 |
| Unit cell angles | 90.00, 118.53, 90.00 |
Refinement procedure
| Resolution | 27.570 - 2.910 |
| R-factor | 0.2318 |
| Rwork | 0.230 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.551 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.570 | 3.010 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.178 | 0.808 |
| Rmeas | 0.211 | 0.954 |
| Rpim | 0.112 | 0.504 |
| Number of reflections | 30932 | 3030 |
| <I/σ(I)> | 7.13 | 1.48 |
| Completeness [%] | 99.4 | 99.84 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.983 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 18% PEG 3350, 0.2M MgCl2 |
