8HRD
Crystal structure of the receptor binding domain of SARS-CoV-2 Delta variant in complex with IMCAS74 Fab and W14 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-08 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.662, 270.048, 118.156 |
| Unit cell angles | 90.00, 107.85, 90.00 |
Refinement procedure
| Resolution | 63.060 - 2.860 |
| R-factor | 0.2126 |
| Rwork | 0.212 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4382) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.470 | 3.010 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmerge | 0.130 | 0.822 |
| Number of reflections | 134730 | 19639 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7 | |
| CC(1/2) | 0.997 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ammonium formate, 20% w/v Polyethylene glycol 3350 |
