8HPY
Crystal structure of human LGI1-ADAM22 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97900 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 280.342, 280.342, 280.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.080 - 5.870 |
| R-factor | 0.305 |
| Rwork | 0.303 |
| R-free | 0.34300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ZXR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.970 |
| High resolution limit [Å] | 5.870 | 5.870 |
| Rmerge | 0.073 | 0.891 |
| Number of reflections | 9884 | 502 |
| <I/σ(I)> | 25.905 | 2.514 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.5M sodium chloride, 0.1M HEPES pH=7.5, 2% (w/v) Benzamidine |
