8HNT

Crystal structure of anti-CRISPR protein AcrIIC4 bound to HpaCas9-sgRNA surveillance complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	205.834, 134.592, 67.531
Unit cell angles	90.00, 105.04, 90.00

Refinement procedure

Resolution	33.220 - 3.060
R-factor	0.2381
Rwork	0.237
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jdq
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.050	3.050
Rmerge	0.080	0.716
Number of reflections	32735	1572
<I/σ(I)>	12.95
Completeness [%]	98.3
Redundancy	3.3
CC(1/2)	0.996	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	tris, sodium chloride, PEG 3000