8HNT

Crystal structure of anti-CRISPR protein AcrIIC4 bound to HpaCas9-sgRNA surveillance complex

Summary for 8HNT

Entry DOI	10.2210/pdb8hnt/pdb
Descriptor	CRISPR-associated endonuclease Cas9, sgRNA, anti-CRISPR protein AcrIIC4 (3 entities in total)
Functional Keywords	cas9, antimicrobial protein, rec lobe, immune system
Biological source	Haemophilus parainfluenzae More
Total number of polymer chains	3
Total formula weight	172649.29
Authors	Sun, W.,Cheng, Z.,Wang, Y. (deposition date: 2022-12-08, release date: 2023-07-19, Last modification date: 2023-08-02)
Primary citation	Sun, W.,Cheng, Z.,Wang, J.,Yang, J.,Li, X.,Wang, J.,Chen, M.,Yang, X.,Sheng, G.,Lou, J.,Wang, Y. AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation. Proc.Natl.Acad.Sci.USA, 120:e2303675120-e2303675120, 2023 Cited by PubMed: 37494395 DOI: 10.1073/pnas.2303675120 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.06 Å)

Structure validation