8HCF
Crystal structure of mTREX1-UMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.031, 85.771, 100.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.143 - 1.600 |
R-factor | 0.1543 |
Rwork | 0.152 |
R-free | 0.17890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yws |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.053 | 0.037 | 0.490 |
Rmeas | 0.057 | 0.040 | 0.526 |
Rpim | 0.020 | 0.014 | 0.191 |
Total number of observations | 558283 | ||
Number of reflections | 73263 | 7648 | 7223 |
<I/σ(I)> | 15.8 | ||
Completeness [%] | 99.9 | 99.2 | 100 |
Redundancy | 7.6 | 7.3 | 7.3 |
CC(1/2) | 0.999 | 0.895 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |