8HCC

Crystal structure of mTREX1-RNA product complex (AMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-22
Detector	RAYONIX MX-300
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	71.197, 86.433, 75.218
Unit cell angles	90.00, 97.60, 90.00

Refinement procedure

Resolution	27.164 - 2.000
R-factor	0.1701
Rwork	0.167
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yws
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.074	0.039	0.477
Rmeas	0.085	0.045	0.551
Rpim	0.042	0.022	0.273
Total number of observations	252782
Number of reflections	61417	6259	6088
<I/σ(I)>	12.2
Completeness [%]	99.9	99.7	99.9
Redundancy	4.1	4.1	4
CC(1/2)		0.997	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 20% w/v Polyethylene glycol 8,000