8H9H

Crystal structure of ZBTB7A in complex with GACCC-containing sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 65 2 2
Unit cell lengths	63.542, 63.542, 201.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.300 - 2.200
R-factor	0.226
Rwork	0.224
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eyi
Data reduction software	XDS
Data scaling software	Aimless (0.7.9)
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.300	48.300	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.131	0.095	2.137
Rmeas	0.133	0.097	2.166
Rpim	0.022	0.019	0.350
Total number of observations		5973	40783
Number of reflections	23116	253	1102
<I/σ(I)>	17.6	33.4	2.3
Completeness [%]	100.0	99.3	100
Redundancy	36	23.6	37
CC(1/2)	0.997	0.994	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2 M sodium chloride, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG 3350