8H62
Crystal structure of Internalin A from Listeria monocytogenes with human E-cadherin EC12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.350, 69.580, 72.490 |
| Unit cell angles | 101.48, 97.77, 105.99 |
Refinement procedure
| Resolution | 34.800 - 1.910 |
| R-factor | 0.1776 |
| Rwork | 0.176 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o6s 4zmt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.371 |
| Data reduction software | MOSFLM (7.2.1) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP (2.7.17) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.800 | 1.950 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.088 | 0.760 |
| Number of reflections | 71483 | 4418 |
| <I/σ(I)> | 8.8 | 2.1 |
| Completeness [%] | 92.3 | 92.1 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.996 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Ammonium acetate 45% MPD |
