8GZ5
Crystal structure of neutralizing VHH P17 in complex with SARS-CoV-2 Alpha variant spike receptor-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 82.680, 82.680, 162.479 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.190 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 71557 | 40098 |
<I/σ(I)> | 12.2 | 0.8 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 10.2 | 10.7 |
CC(1/2) | 0.999 | 0.545 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Bis-Tris propane pH 7.0, 1.0M succinic acid pH 7.0 |