8GUW

Structure of Aurora Kinase A in complex with activator peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	64.859, 78.695, 185.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.980 - 2.700
R-factor	0.2159
Rwork	0.212
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dt3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rpim	0.030	0.170
Number of reflections	26742	2635
<I/σ(I)>	16.625
Completeness [%]	99.6
Redundancy	9.4
CC(1/2)	0.991	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291	0.1M Tris pH 8.0, 0.2M lithium sulfate, 15% (w/v) polyethylene glycol 3350