8GSI

Structure of the cobolimab Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-22
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	64.300, 119.300, 184.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.830 - 2.020
R-factor	0.1989
Rwork	0.197
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ggs
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.080
High resolution limit [Å]	2.020	2.020
Rmeas	0.154
Number of reflections	46872	2340
<I/σ(I)>	7.1
Completeness [%]	99.9
Redundancy	5.7
CC(1/2)	0.987	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1 M Sodium cacodylate pH 6.5 40% v/v MPD 5% w/v PEG 8000