8GQ1
HyHEL10 Fab complexed with hen egg lysozyme carrying arginine cluster in framework region of light chain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 147.900, 147.900, 137.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.800 - 3.130 |
R-factor | 0.2314 |
Rwork | 0.230 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d9a |
RMSD bond length | 0.004 |
RMSD bond angle | 0.883 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 3.240 |
High resolution limit [Å] | 3.130 | 3.130 |
Rmerge | 0.090 | 0.575 |
Rmeas | 0.128 | 0.813 |
Rpim | 0.090 | 0.575 |
Number of reflections | 16124 | 1566 |
<I/σ(I)> | 8.86 | 1.08 |
Completeness [%] | 99.3 | 99.94 |
Redundancy | 2 | 2 |
CC(1/2) | 0.987 | 0.545 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 400, sodium citrate tribasic dihydrate, Tris hydrochloride |