8GHP
GUCY2C-ECD bound to anti-GUCY2C-scFv antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-09-25 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 199.629, 199.629, 123.267 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.210 - 3.520 |
| R-factor | 0.275 |
| Rwork | 0.274 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.770 |
| High resolution limit [Å] | 3.520 | 3.520 |
| Rmerge | 0.120 | 0.900 |
| Number of reflections | 17636 | 881 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 91.8 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2M ammonium H2-PO4, 100mM Tris hydrochloride at pH 8.5 |
