8GAE
Hsp90 provides platform for CRaf dephosphorylation by PP5
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.3 |
Number of Particle | 3730385 |
Software | cryoSPARC,RELION,UCSF ChimeraX,SerialEM,CTFFIND |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |