8G8F
Human IMPDH2 mutant - L245P, treated with ATP, IMP, and NAD+; extended filament segment reconstruction
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.6 |
Number of Particle | 1893802 |
Software | RELION,PHENIX,Coot,ISOLDE,Leginon |
Symmetry | D4 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |