8G0M
Structure of complex between TV6.6 and CD98hc ECD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-08-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.18071 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 136.152, 167.126, 83.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.250 |
| Rwork | 0.204 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6irs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.219 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.681 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.054 | |
| Rmeas | 0.058 | 1.070 |
| Number of reflections | 45610 | 4148 |
| <I/σ(I)> | 22.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 10% Glycerol, 0.1 M HEPES pH 7.5, 5% PEG 3000, 30% PEG 40 |
