8FT8
The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Thr-Val linker variant, SUMO tag-free preparation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979460 |
Spacegroup name | P 65 |
Unit cell lengths | 100.882, 100.882, 49.754 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.870 - 1.600 |
R-factor | 0.1833 |
Rwork | 0.182 |
R-free | 0.20420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qar 1shu |
RMSD bond length | 0.006 |
RMSD bond angle | 0.758 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.870 | 1.658 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.056 | 0.990 |
Rmeas | 0.058 | |
Rpim | 0.013 | 0.225 |
Number of reflections | 38098 | 3766 |
<I/σ(I)> | 25.28 | |
Completeness [%] | 99.6 | 99.13 |
Redundancy | 19 | |
CC(1/2) | 0.999 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M HEPES, 2.0M ammonium sulfate |