8FT6
The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Ala-Ala linker variant, SUMO tag-free preparation.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 99.745, 99.745, 50.135 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.190 - 2.620 |
R-factor | 0.2063 |
Rwork | 0.205 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.567 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.190 | 2.714 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.117 | |
Rmeas | 0.119 | |
Rpim | 0.020 | |
Number of reflections | 8712 | 861 |
<I/σ(I)> | 27.46 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 34.1 | 33 |
CC(1/2) | 1.000 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2M Ammonium citrate tribasic, 20% w/v PEG 3350 |