8FT6

The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Ala-Ala linker variant, SUMO tag-free preparation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	99.745, 99.745, 50.135
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.190 - 2.620
R-factor	0.2063
Rwork	0.205
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.567
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.190	2.714
High resolution limit [Å]	2.620	2.620
Rmerge	0.117
Rmeas	0.119
Rpim	0.020
Number of reflections	8712	861
<I/σ(I)>	27.46
Completeness [%]	99.9	100
Redundancy	34.1	33
CC(1/2)	1.000	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2M Ammonium citrate tribasic, 20% w/v PEG 3350