8FMK
Crystal structure of human KRAS with extended switch I loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-11-11 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.643, 47.653, 90.101 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.120 - 1.480 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5us4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.818 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.650 | 1.510 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmeas | 0.075 | 1.012 |
Rpim | 0.028 | 0.431 |
Number of reflections | 24873 | 1155 |
<I/σ(I)> | 18.5 | 1.6 |
Completeness [%] | 99.7 | 96 |
Redundancy | 6.7 | 5.2 |
CC(1/2) | 0.999 | 0.668 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | (NH)4Cl 0.2 M PEG3350 20-24% |