8FJ3

Crystal Structure of the Tick Evasin EVA-AAM1001 Complexed to Human Chemokine CCL7(Y13A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	P 41 21 2
Unit cell lengths	72.272, 72.272, 65.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.300 - 2.070
R-factor	0.2143
Rwork	0.213
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.675
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	2.130
High resolution limit [Å]	2.070	2.070
Number of reflections	11114	846
<I/σ(I)>	14.1
Completeness [%]	99.9
Redundancy	24.5
CC(1/2)	0.998	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2 M NH2SO4, 0.1 Na acetate, pH 4.8