8F8W

Crystal structure of Nb.X0 bound to the afucosylated human IgG1 fragment crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 61
Unit cell lengths	170.636, 170.636, 126.419
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.900 - 2.710
R-factor	0.2088
Rwork	0.208
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.178
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.710	2.710
Rpim	0.035	0.522
Number of reflections	56766	2863
<I/σ(I)>	31.1	1.5
Completeness [%]	98.2
Redundancy	4.6
CC(1/2)	1.000	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium HEPES 7.5 20 % w/v PEG 8000. Cryopreserved with 12.5% glycerol.