8F8W
Crystal structure of Nb.X0 bound to the afucosylated human IgG1 fragment crystal form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.92010 |
Spacegroup name | P 61 |
Unit cell lengths | 170.636, 170.636, 126.419 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.900 - 2.710 |
R-factor | 0.2088 |
Rwork | 0.208 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.178 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.710 | 2.710 |
Rpim | 0.035 | 0.522 |
Number of reflections | 56766 | 2863 |
<I/σ(I)> | 31.1 | 1.5 |
Completeness [%] | 98.2 | |
Redundancy | 4.6 | |
CC(1/2) | 1.000 | 0.604 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium HEPES 7.5 20 % w/v PEG 8000. Cryopreserved with 12.5% glycerol. |