8F8M

LRH-1 bound to small molecule Tet and fragment of coactivator Tif2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-02-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	89.161, 89.161, 105.692
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.580 - 2.600
R-factor	0.2033
Rwork	0.196
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dos
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.600	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	15066	1235
<I/σ(I)>	12.1	1.38
Completeness [%]	97.7
Redundancy	11.2
CC(1/2)	0.937	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	tri-Na citrate, tert-butanol, glycerol